A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells.
نویسندگان
چکیده
In a clinical study of 190 men with non-gonococcal urethritis, Chlamydia trachomatis inclusions were sought in cycloheximide-treated McCoy cells by an indirect immunofluorescent antibody technique. The method was consistently reliable over a period of two years, and the results were obtained within 24 hours of a patient's attendance. The results correlated with those obtained by Giemsa staining in 91.6% of patients, and the new method was at least as sensitive as the established Giemsa-staining method.
منابع مشابه
Detection of Chlamydia trachomatis inclusions in McCoy and HeLa-229 cells: an alternative staining technique using toluidine blue.
Toluidine blue staining was used to detect Chlamydia trachomatis inclusions in both McCoy and HeLa-229 cells from clinical specimens. This method was more sensitive than iodine staining for detecting C trachomatis inclusions in both McCoy and HeLa-229 cells and also more sensitive than Giemsa staining for detecting chlamydial inclusions in HeLa-229 cells. While its sensitivity for detection of ...
متن کاملبررسی قدرت تشخیصی روشهای ایمونوفلئورسانس مستقیم( (DFAو رنگ آمیزی گیمسا در مقایسه با روش PCR ژن Omp1 جهت شناسایی کلامیدیا تراکوماتیس در نمونه ملتحمه بیماران مبتلا به کونژنکتیویت فولیکولار مراجعه کننده به بیمارستان فارابی در سال 1392
Background and Aim: Chlamydia trachomatis is an obligate intracellular parasite responsible for ocular and genital infections in human. Chlamydia trachomatis is the most common cause of chronic follicular conjunctivitis and is also responsible for 20% of acute conjunctivitis cases. As a rapid diagnosis is important in the reducing the long-term squeal of the diseases, the objective of this stud...
متن کاملSensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of a fluorescein labelled monoclonal antibody: comparison with conventional chlamydial isolation.
Commercially produced fluorescein labelled monoclonal antibodies for the detection of Chlamydia trachomatis have recently become available. One is for detecting inclusions in cell culture (culture confirmation) and the other for detecting elementary bodies in smears from potentially infected sites. We have compared the two monoclonal antibodies with our routine isolation method, which utilises ...
متن کاملA rapid method for staining inclusions of Chlamydia psittaci and Chlamydia trachomatis.
A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale green. The method was significantly better than Giemsa staining and comparable to...
متن کاملSensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture.
Monoclonal antibodies which recognize the species-specific major outer membrane protein antigen of Chlamydia trachomatis were used for immunofluorescence staining of chlamydial inclusions in cell culture. A total of 115 clinical specimens were inoculated onto replicate HeLa 229 cell monolayers and assayed for chlamydial inclusions by immunofluorescence staining and Giemsa staining. Of the isola...
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 33 2 شماره
صفحات -
تاریخ انتشار 1980